Flavonoids from Millettia nitida var. hirsutissima with their anticoagulative activities and inhibitory effects on NO production
| Autor: | Xiao-Lin Liao, Jian-Guang Luo, Ling-Yi Kong |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Stereochemistry Inhibitory postsynaptic potential Nitric Oxide Millettia Nitric oxide Cell Line chemistry.chemical_compound Ic50 values medicine Animals No production Flavonoids Traditional medicine biology Macrophages Antithrombin Millettia nitida Anticoagulants Antithrombin Activity biology.organism_classification chemistry Molecular Medicine Rabbits medicine.drug |
| Zdroj: | Journal of natural medicines. 67(4) |
| ISSN: | 1861-0293 |
| Popis: | Two new compounds, (2R)-7,3′-dihydroxy-6,4′-methoxyflavan (1) and (3R)-7,4′-dihydroxy-2′-methoxyisoflavan (2), along with 12 known flavonoids (3–14), were isolated from the vine stems of Millettia nitida var. hirsutissima. The structures of the isolated compounds were elucidated based on spectroscopic analyses and comparison of literature data. All of the isolates were evaluated for their effects on in-vitro anticoagulative assay and on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW264.7 macrophages. As a result, all compounds showed weak antiplatelet aggregation activities and compounds 4, 5, 7, 8 and 9 demonstrated antithrombin activity with a good dose–effect relationship from 5 to 100 μg mL−1 in rabbit plasma. Compounds 8, 9, 10 and 14 exhibited inhibitory effects on LPS-induced NO production in RAW264.7 macrophages with IC50 values of 4.79 ± 0.20, 8.23 ± 0.35, 5.23 ± 0.11 and 6.30 ± 0.30 μg mL−1, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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