Impact of Ecklonia stolonifera extract on in vitro ruminal fermentation characteristics, methanogenesis, and microbial populations
| Autor: | Nyeon Hak Shin, Shin Ja Lee, Sung Sill Lee, Hyun Sang Kim, Su Kyoung Lee, Jun Sik Eom, Jin Suk Jeong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Methanogenesis
Population lcsh:Animal biochemistry Ecklonia stolonifera Extract Article Ruminant Nutrition and Forage Utilization Rumen Microbial Population Food science extract education fermentation lcsh:QP501-801 In vitro Fermentation lcsh:SF1-1100 education.field_of_study Fibrobacter succinogenes biology Chemistry Methane Emission biology.organism_classification Stolonifera Hay Animal Science and Zoology Fermentation lcsh:Animal culture Ecklonia stolonifera Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences Asian-Australasian Journal of Animal Sciences, Vol 32, Iss 12, Pp 1864-1872 (2019) |
| ISSN: | 1976-5517 1011-2367 |
| Popis: | Objective This study was conducted to evaluate the effects of Ecklonia stolonifera (E. stolonifera) extract addition on in vitro ruminal fermentation characteristics, methanogenesis and microbial populations. Methods One cannulated Holstein cow (450±30 kg) consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (09:00 and 17:00) at 2% of body weight with free access to water and mineral block were used as rumen fluid donors. In vitro fermentation experiment, with timothy hay as substrate, was conducted for up to 72 h, with E. stolonifera extract added to achieve final concentration 1%, 3%, and 5% on timothy hay basis. Results Administration of E. stolonifera extract to a ruminant fluid-artificial saliva mixture in vitro increased the total gas production. Unexpectedly, E. stolonifera extracts appeared to increase both methane emissions and hydrogen production, which is contrasts with previous observations with brown algae extracts used under in vitro fermentation conditions. Interestingly, real-time polymerase chain reaction indicated that as compared with the untreated control the ciliate-associated methanogen and Fibrobacter succinogenes populations decreased, whereas the Ruminococcus flavefaciens population increased as a result of E. stolonifera extract supplementation. Conclusion E. stolonifera showed no detrimental effect on rumen fermentation characteristics and microbial population. Through these results E. stolonifera has potential as a viable feed supplement to ruminants. |
| Databáze: | OpenAIRE |
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