Molecular cloning and characterization of GuHMGR, an HMG-CoA reductase gene from liquorice (Glycyrrhiza uralensis)
| Autor: | Chunsheng Liu, Wenquan Wang, Chunying Ma |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
biology
Glycyrrhiza uralensis cloning food and beverages Molecular cloning biology.organism_classification Protein tertiary structure Medicago truncatula Homology (biology) Open reading frame genome walker HMG-CoA reductase Biochemistry Primer walking liquorice Agronomy and Crop Science Gene glycyrrhiza uralensis Research Article |
| Zdroj: | Frontiers of Agriculture in China |
| ISSN: | 1673-744X 1673-7334 |
| Popis: | A full length cDNA encoding HMGR (designated as GuHMGR) was isolated from liquorice (Glycyrrhiza uralensis) based on degenerated PCR and genome walking. The full length cDNA of GuHMGR was 2330 bp with a 1518-bp open reading frame (ORF) encoding a 505-aa polypeptide. Bioinformatics analysis indicated that there were two trans-membrane domains in GuHMGR. A molecular model of tertiary structure showed that GuHMGR is a novel HMGR with a similar spatial structure to other plant HMGRs. The deduced polypeptide of GuHMGR has an isoelectric point (pI) of 6.41 and a calculated molecular weight of about 54.7 kDa. Sequence comparison and phylogenetic tree analysis showed that GuHMGR had the highest homology with HMGRs from Pisum sativum and Medicago truncatula, indicating that GuHMGR belongs to the plant HMGR group. Expression analysis showed the similar amount of transcript level of GuHMGR in roots and leaves, suggesting that this gene was expressed constitutively in plants. Therefore, this novel HMGR gene would possibly provide a new strategy for studying the glycyrrhizin metabolism at the molecular level in the future. |
| Databáze: | OpenAIRE |
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