Presence and active synthesis of the 67 kDa elastin-receptor in human circulating white blood cells
| Autor: | Ladislas Robert, Danièle Gagné, Anis Larbi, Georges Levesque, Tamas Fulop, Nadine Douziech |
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| Rok vydání: | 2005 |
| Předmět: |
Cell type
Neutrophils T-Lymphocytes Cell Biophysics Gene Expression Receptors Cell Surface In Vitro Techniques Biochemistry Immune system Gene expression Leukocytes medicine Humans RNA Messenger Molecular Biology Messenger RNA biology Mesenchymal stem cell Cell Biology Molecular biology Elastin Molecular Weight Protein Subunits medicine.anatomical_structure biology.protein Antibody |
| Zdroj: | Biochemical and Biophysical Research Communications. 332:787-792 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2005.05.021 |
| Popis: | Early after the identification of the elastin-receptor (El-R) on mesenchymal cells, it was demonstrated that phagocytic cells and lymphocytes could also respond to elastin peptides. Nevertheless, the level of El-R expression has never been demonstrated on immune cells and no data exist whether these cells actively synthesize this El-R. Thus, our aim in the present work was to study the expression and number of El-R on white blood cells (WBC) using a specific 67 kDa El-R antibody and to demonstrate the presence of mRNA corresponding to the gene coding for El-R. Our results show that messenger RNA corresponding to the presumptive gene coding for the 67 kDa El-R subunit could be detected in all three WBC-types investigated. On all of these WBC, the presence of El-R could be demonstrated, however their number and their function varied following the cell type. The presence of El-R is very important for the interaction of circulating cell with the matrix as these cells intervene during atherosclerosis and in host defence. |
| Databáze: | OpenAIRE |
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