Effect of kisspeptin-54 on ovarian levels of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in an experimental model of ovarian hyperstimulation syndrome (OHSS)
| Autor: | Hülya Birinci, Mehmet Yüncü, Hafize Seda Vatansever |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Ovarian hyperstimulation syndrome Controlled ovarian hyperstimulation Reproductive technology Biology Rats Sprague-Dawley Ovarian Hyperstimulation Syndrome chemistry.chemical_compound Endocrinology PEDF Internal medicine Genetics medicine Animals Nerve Growth Factors Eye Proteins Protein kinase A Ovarian reserve Molecular Biology Serpins Protein kinase C Kisspeptins Ovary medicine.disease Rats Vascular endothelial growth factor Disease Models Animal Reproductive Medicine chemistry Female Animal Science and Zoology hormones hormone substitutes and hormone antagonists Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 33:799-809 |
| ISSN: | 1448-5990 1031-3613 |
| DOI: | 10.1071/rd21140 |
| Popis: | The purpose of this study was to investigate the effect of kisspeptin-54 on ovarian morphology and vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), protein kinase A (PKA) and protein kinase C (PKC) levels in an ovarian hyperstimulation syndrome (OHSS) rat model, which is a possible complication of controlled ovarian hyperstimulation. For this purpose, immature female Sprague–Dawley rats (25 days old, 30–40 g) were randomly divided into five groups (control, sham, OHSS model, short-term kisspeptin-54 administered OHSS model and long-term kisspeptin-54 administered OHSS model). Serum LH and FSH levels were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and quantitative RT-PCR were performed for VEGF, PEDF, PKA and PKC in ovaries and granulosa cells, respectively. It was observed that there was dilatation in fallopian tubes and an abnormal increase in ovarian weight and volume in the OHSS group, and these morphologies decreased with kisspeptin-54 treatment. After the administration of kisspeptin-54 in the OHSS group, VEGF, PKA and PKC levels reduced and PEDF levels increased in both mRNA and protein levels. It was determined that in the OHSS model, VEGF increased as PEDF decreased, and kisspeptin-54 reduced the effects of OHSS. It was determined that long-term kisspeptin-54 treatment was more effective than short-term administration. It is considered that kisspeptin-54 is an agent that protects ovarian reserve and oocyte maturation in women at risk of OHSS. |
| Databáze: | OpenAIRE |
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