IGF-I stimulates chemotaxis of human neuroblasts. Involvement of type 1 IGF receptor, IGF binding proteins, phosphatidylinositol-3 kinase pathway and plasmin system
| Autor: | Antonella Puglianiello, Stefano Cianfarani, Daniela Germani, Paolo Rossi |
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| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Motility Biology Receptor IGF Type 1 Wortmannin Phosphatidylinositol 3-Kinases chemistry.chemical_compound Insulin-like growth factor Endocrinology Neuroblast Neuroblast migration Internal medicine medicine Humans Insulin-Like Growth Factor I Receptor Cells Cultured Phosphoinositide-3 Kinase Inhibitors Neurons Chemotaxis Antibodies Monoclonal Plasminogen Cell migration Insulin-Like Growth Factor Binding Proteins Plasminogen Inactivators chemistry Signal transduction |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | SH-SY5Y human neuroblastoma cells express IGF receptors, IGFs and IGF binding proteins (IGFBPs), and provide a model for studying the role of the IGF system in human neuronal development. We investigated the effect of IGF-I and des(1-3)IGF-I on the motility of SH-SY5Y cells by a cell migration assay based on the assessment of the number of cells which migrated across 8 microm pore size membranes and around an agarose drop. IGF-I and des(1-3)IGF-I stimulated neuroblast chemotaxis in a dose-dependent manner. Treatment of cells with these agents for 24 h resulted in a significant increase (IGF-I by 70% and des(1-3)IGF-I by 90%; P |
| Databáze: | OpenAIRE |
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