Robotic solid phase extraction and high performance liquid chromatographic analysis of ranitidine in serum or plasma
| Autor: | Ann E. Gooding, Thomas L. Lloyd, Teresa B. Perschy, Julie J. Tomlinson |
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| Rok vydání: | 1992 |
| Předmět: |
Quality Control
Clinical Biochemistry Analytical chemistry Biological Availability Procainamide Ranitidine Biochemistry High-performance liquid chromatography Analytical Chemistry Drug Discovery medicine Humans Solid phase extraction Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Chemistry General Medicine Plasma Robotics Reference Standards Robotic systems Fully automated Spectrophotometry Ultraviolet medicine.drug |
| Zdroj: | Biomedical chromatography : BMC. 6(6) |
| ISSN: | 0269-3879 |
| Popis: | A fully automated assay for the analysis of ranitidine in serum and plasma, with and without an internal standard, was validated. It utilizes robotic solid phase extraction with on-line high performance liquid chromatographic (HPLC) analysis. The ruggedness of the assay was demonstrated over a three-year period. A Zymark Py Technology II® robotic system was used for serial processing from initial aspiration of samples from original collection containers, to final direct injection onto the on-line HPLC system. Automated serial processing with on-line analysis provided uniform sample history and increased productivity by freeing the chemist to analyse data and perform other tasks. The solid phase extraction efficiency was 94% throughout the assay range of 10–250 ng/mL. The coefficients of variation for within- and between-day quality control samples ranged from 1 to 6% and 1 to 5%, respectively. Mean accuracy for between-day standards and quality control results ranged from 97 to 102% of the respective theoretical concentrations. |
| Databáze: | OpenAIRE |
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