Cortisol increases transfection efficiency of cells
| Autor: | Brigitte M. Frey, Emanuela Lovati, Alexandre G Rebuffat, Felix J. Frey, Alessio G.F Bernasconi, Ivo Galli |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.medical_specialty
Hydrocortisone Biophysics DNA Recombinant Glucocorticoid receptor Biology Transfection Biochemistry Dexamethasone Cell Line 03 medical and health sciences 0302 clinical medicine Receptors Glucocorticoid Structural Biology Internal medicine Genetics medicine Animals Humans Receptor Molecular Biology Glucocorticoids Gene transfer 030304 developmental biology 0303 health sciences Reporter gene fungi Cell Biology Rats Dissociation constant Cortisone Mifepristone Endocrinology 11β-glucocorticosteroid Cell culture 030220 oncology & carcinogenesis Glucocorticoid hormones hormone substitutes and hormone antagonists medicine.drug HeLa Cells |
| Zdroj: | FEBS Letters. (1):103-106 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(97)01439-7 |
| Popis: | DNA uptake can be facilitated by addition of physiological amounts of 11β-hydroxy glucocorticosteroids (such as cortisol) during transfection. In the presence of cortisol, but not of the inactive 11-keto glucocorticoid cortisone, twice as many cells uptake and express the reporter gene. The effect is specific and dose-dependent; the amounts of glucocorticosteroids needed to enhance transfection efficiency are in the nanomolar range, which corresponds to the dissociation constant of glucocorticoids for the glucocorticoid receptor in vitro. This effect can be abolished by an excess of the glucocorticoid antagonist RU486. We infer that the activated cytoplasmic glucocorticoid receptors enhance nuclear translocation of the incoming transfected DNA. |
| Databáze: | OpenAIRE |
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