Dust exposure and pulmonary inflammation in Standardbred racehorses fed dry hay or haylage: A pilot study
Autor: | Janice E. Kritchevsky, Abhijit Mukhopadhyay, K.M. Ivester, Carla Olave, Stacy H. Tinkler, Laurent L. Couëtil |
---|---|
Rok vydání: | 2020 |
Předmět: |
beta-Glucans
040301 veterinary sciences Neutrophils Pilot Projects 030204 cardiovascular system & hematology Poaceae 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Animal science Cytology medicine Animals Horses Treadmill Asthma General Veterinary medicine.diagnostic_test business.industry Horse Dust 04 agricultural and veterinary sciences Environmental Exposure Pneumonia medicine.disease Animal Feed Neutrophilia respiratory tract diseases Endotoxins Bronchoalveolar lavage medicine.anatomical_structure Hay Cytokines Animal Science and Zoology Horse Diseases medicine.symptom business Bronchoalveolar Lavage Fluid Respiratory tract Medicago sativa |
Zdroj: | Veterinary journal (London, England : 1997). 271 |
ISSN: | 1532-2971 |
Popis: | Respirable dust exposure is linked to airway inflammation in racehorses. Feeding haylage may reduce dust exposure by 60-70%. The objective of this study was to compare dust exposure, airway cytology, and inflammatory cytokine concentrations between horses fed haylage or hay over 6 weeks while in training. Seven healthy Standardbred horses were randomly assigned to be fed alfalfa hay (n = 3) or grass-alfalfa mix haylage (n = 4) for six weeks while training on a treadmill. Dust exposure was measured gravimetrically at the breathing zone. Endotoxin and β-glucan concentrations in respirable dust were measured. Bronchoalveolar lavage fluid (BALF) cytology was determined at baseline and after 2, 4, and 6 weeks. Cytokine concentrations (interferon-γ, tumor necrosis factor-α, and interleukin-4) were measured in BALF at baseline and week 6. The effect of forage on exposure, airway cytology and cytokines were evaluated using generalized linear mixed models. Respirable dust and β-glucan exposures were lower in horses fed haylage than hay (0.02 ± 0.001 mg/m3 vs. 0.06 ± 0.01 mg/m3; P = 0.03, and 69 ± 18 pg/m3 vs. 160 ± 21 pg/m3; P = 0.02, respectively). In horses eating haylage, BALF neutrophil proportion decreased between baseline (2.2 ± 0.5%), week 2 (0.8 ± 0.3%; P = 0.01) and week 6 (0.7 ± 0.2%; P = 0.03). By week 6, horses fed haylage had lower BALF neutrophilia than horses fed hay (4.0 ± 0.7 %; P = 0.0004). Interleukin-4 concentration in BALF was higher at week 6 (14.4 ± 4.6 pg/mL) in horses fed hay compared to baseline (2.9 ± 4.6 pg/mL; P = 0.007). In conclusion, feeding haylage instead of hay to horses in training can reduce exposure to respirable irritants and mitigate airway neutrophilia. |
Databáze: | OpenAIRE |
Externí odkaz: |