Microbial levan in the diet ofLabeo rohitaHamilton juveniles: effect on non-specific immunity and histopathological changes after challenge withAeromonas hydrophila
| Autor: | Vikas Kumar, Sanjay Kumar Gupta, S.C. Mukherjee, Narottam Prasad Sahu, Rishikesh S. Dalvi, Asim K. Pal |
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| Rok vydání: | 2008 |
| Předmět: |
Globulin
medicine.drug_class Veterinary (miscellaneous) Cyprinidae Aquatic Science Kidney Immunostimulant Microbiology Fish Diseases Basal (phylogenetics) chemistry.chemical_compound Animal science medicine Animals Respiratory Burst biology Probiotics Albumin biology.organism_classification Survival Analysis Immunity Innate Aeromonas hydrophila Diet Fructans Labeo medicine.anatomical_structure Liver chemistry Hepatocytes biology.protein Muramidase Lysozyme Gram-Negative Bacterial Infections |
| Zdroj: | Journal of Fish Diseases. 31:649-657 |
| ISSN: | 1365-2761 0140-7775 |
| DOI: | 10.1111/j.1365-2761.2008.00939.x |
| Popis: | A 60-day feeding trial was conducted to study the immuno-protective effect of microbial levan on Labeo rohita juveniles challenged with Aeromonas hydrophila. Six purified diets were prepared with different levels of microbial levan: control (no levan), T1 (Basal + 0.25%), T2 (Basal + 0.50%), T3 (Basal + 0.75%), T4 (Basal + 1%) and T5 (Basal + 1.25%), fed to six groups of fish in triplicate. Among the treatment groups the haemoglobin content and total leucocyte count were increased with a dietary supplementation of levan at 1% or more. An increasing trend for total erythrocyte count was observed with increasing level of dietary levan. Lower levan-supplemented groups showed a higher albumin/globulin ratio. As the levan supplementation was increased, there was a gradual increase in serum lysozyme activity and respiratory burst activity [nitroblue tetrazolium (NBT) assay] reduction values. The highest lysozyme activity and NBT were observed in the T5 group although this was similar to the T4 group (P > 0.05). No significant histo-architectural changes were associated with dietary levan levels. After challenge with A. hydrophila, moderately degenerated hepatocytes, oedema and leucocytic infiltration in parenchymatous tissues, and extensive haemorrhage and haemosiderosis in the kidney were observed in the control group. However, the T5 group supplemented with 1.25% levan showed infiltrating leucocytes in the liver while the kidney showed only moderate degeneration of renal tubules. The relative survival per cent of juveniles after challenge with A. hydrophila was the highest in the T5 group followed by T4. This suggests that microbial levan at 1.25% can be used as dietary immunostimulant for L. rohita juveniles. |
| Databáze: | OpenAIRE |
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