Inhibition of pulmonary metastasis by Emilia sonchifolia (L.) DC: An in vivo experimental study

Autor: Gilcy George K, Girija Kuttan
Rok vydání: 2016
Předmět:
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
Pathology
medicine.medical_specialty
Lung Neoplasms
medicine.medical_treatment
Melanoma
Experimental
India
Pharmaceutical Science
Asteraceae
Pharmacology
Matrix metalloproteinase
Metastasis
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Emilia sonchifolia
In vivo
Drug Discovery
Animals
Medicine
Neoplasm Metastasis
Extracellular Signal-Regulated MAP Kinases
Interleukin 6
Lung
Plants
Medicinal
biology
Plant Extracts
business.industry
Granulocyte-Macrophage Colony-Stimulating Factor
biology.organism_classification
medicine.disease
Matrix Metalloproteinases
Mice
Inbred C57BL
Vascular endothelial growth factor
Vascular endothelial growth factor A
030104 developmental biology
Cytokine
Complementary and alternative medicine
chemistry
030220 oncology & carcinogenesis
biology.protein
Cytokines
Molecular Medicine
business
Zdroj: Phytomedicine. 23:123-130
ISSN: 0944-7113
DOI: 10.1016/j.phymed.2015.11.017
Popis: Background Emilia sonchifolia (L.) DC is a widely distributed medicinal herb used mainly in the indigenous Ayurvedic system of medicine in India. This plant is one among the ten sacred plants of Kerala state in India, collectively known as Dasapushpam. Purpose To assess the therapeutic efficacy of this well-known medicinal plant in a catastrophic complication like metastatic cancer progression. This study further aimed to scientifically validate the traditional medicinal use of this sacred plant. Study design Highly metastatic B16F10 melanoma will spontaneously metastasize in C57BL/6 mice and is accepted as a useful murine model for the study on metastasis. Three different experimental modalities of prophylactic, simultaneous and after tumour development were used for data accumulation and analysis. Methods Whole plant genuine extract of E. sonchifolia (25 mg/kg bodyweight) was administered intraperitoneally to C57BL/6 mice. Animals were sacrificed on 21st day after tumour induction and the lung tumour nodules were counted. Various lung and serum biochemical parameters along with major cytokine levels were recorded. Survival rate was monitored. Histopathology of the lung tissue and expression studies of the major genes involved in metastasis was also carried out. Results E. sonchifolia significantly inhibited pulmonary tumour formation and increased the life span of animals. Lung collagen hydroxyproline, uronic acid, hexosamine, serum sialic acid, γ-glutamyl transpeptidase, vascular endothelial growth factor (VEGF), granulocyte monocyte colony-stimulating factor and other cytokine levels were significantly lowered in the treated group of animals. Histopathological analysis was also correlated with these findings. E. sonchifolia down regulated the expression of matrix metalloproteinases; extracellular signal-regulated kinases and VEGF at the same time up regulated the expression of tissue inhibitor of matrix metalloproteinases. Conclusion Previous studies on E. sonchifolia proved its significant biological properties including anti-tumour, anti-inflammatory and antioxidant activities. Present report is so far the first study to demonstrate the anti-metastatic potential of this medicinal herb justifying its conventional use in the traditional medicine.
Databáze: OpenAIRE
Externí odkaz: