Methylopila jiangsuensis sp. nov., an aerobic, facultatively methylotrophic bacterium
| Autor: | Lian Li, Jin-Wei Zheng, Jian He, Shunpeng Li, Yuri A. Trotsenko, Nina V. Doronina, Bao-Jian Hang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Methylopila capsulata
DNA Bacterial China Stereochemistry Sequence analysis Ubiquinone Molecular Sequence Data Biology Microbiology RNA Ribosomal 16S Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Base Composition Sewage Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA biology.organism_classification Methylopila jiangsuensis Biochemistry Methylopila helvetica Energy source Methylocystaceae Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 61(Pt 7) |
| ISSN: | 1466-5034 |
| Popis: | The taxonomic status was determined of an aerobic, facultatively methylotrophic strain, JZL-4T, isolated from activated sludge. The cells were Gram-negative, asporogenous, colourless, motile, short rods. The strain utilized methanol, methylamine, formate and a variety of polycarbon compounds, but not methane, dichloromethane or CO2/H2, as carbon and energy sources. C1 compounds were assimilated via the isocitrate lyase-negative serine pathway. Optimal growth occurred at 30 °C, pH 6.5–7.5 and 0.5 % (w/v) NaCl. The major cellular fatty acids were C18 : 1ω7c and C18 : 0. The major phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine (PME); PME, the main phospholipid of strain JZL-4T, was absent or present in only minor amounts in Methylopila capsulata IM1T, Methylopila helvetica DM9T and Albibacter methylovorans DM10T. The major ubiquinone was Q-10. The DNA G+C content of strain JZL-4T was 70.4 mol% (T m). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain showed high sequence similarities to M. capsulata IM1T (97.2 %), A. methylovorans DM10T (94.9 %) and M. helvetica DM9T (94.1 %), and showed less than 94 % similarity to strains of other species with validly published names. Strain JZL-4T had a low level of DNA–DNA relatedness (34 %) with M. capsulata IM1T. On the basis of phenotypic, genetic and phylogenetic data, strain JZL-4T is proposed to represent a novel species of the genus Methylopila, with the name Methylopila jiangsuensis sp. nov. The type strain is strain JZL-4T ( = ACCC 05406T = DSM 22718T = VKM B-2555T). |
| Databáze: | OpenAIRE |
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