| Autor: |
Robert, Madalen, Waldhauer, Julian, Stritt, Fabian, Yang, Bo, Pauly, Markus, Voiniciuc, C��t��lin |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.14812344.v1 |
| Popis: |
Additional file 1: Figure S1. Sanger sequencing alignments of assembled CSL constructs. Figure S2. Genotyping of domain-swapped AtCSLA2/AkCSLA3 yeast colonies. Figure S3. Screening of Pichia colonies expressing recombinant proteins. Table S1. Complete glycosidic linkage table for alkaline-insoluble polymers. Table S2. Enrichment and composition of (gluco)mannan for the top CSLA strains. Figure S4. Oligosaccharide profiling of commercial (gluco)mannan polysaccharides. Figure S5. Yeast cell density and area after CSLA expression. Figure S6. Imaging and quantification of yeast cells stained with Congo Red. Figure S7. Expression of AkCSLA3 in Nicotiana beanthamiana leaves. Figure S8. Replacement of a CSLA catalytic domain with that of a CSLC. Figure S9. Combinatorial effect of AkCSLA3/AtCSLA2 domain swaps. Figure S10. Alignment of active mannan and/or glucomannan synthases. Figure S11. Alignment of CesAs and CSL representatives. Table S3. Primer sequences used in this study. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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