Inhibitory and modulatory inputs to the vocal central pattern generator of a teleost fish

Autor: Elisabeth Rosner, Kevin N. Rohmann, Boris P. Chagnaud, Andrew H. Bass
Rok vydání: 2017
Předmět:
0301 basic medicine
RRID:AB_10013381
RRID:AB_142672
Connexin
Connexins
GABA
0302 clinical medicine
Neuromodulation
RRID:AB_141708
RRID:AB_2079751
RRID:AB_10718516
Research Articles
Catecholaminergic
Neurons
Neurotransmitter Agents
RRID:AB_572268
General Neuroscience
RRID:AB_94632
Central pattern generator
Gap Junctions
RRID:AB_2560949
inhibition
serotonin
medicine.anatomical_structure
CpG site
neuromodulation
GABAergic
RRID:AB_2340846
dopamine
Research Article
Tyrosine 3-Monooxygenase
RRID:AB_2337244
Glycine
Biotin
Hindbrain
Biology
Inhibitory postsynaptic potential
Statistics
Nonparametric
Choline O-Acetyltransferase
gap junction
03 medical and health sciences
vocal pattern generation
otorhinolaryngologic diseases
medicine
Animals
teleost fish
Neural Inhibition
Batrachoidiformes
030104 developmental biology
Central Pattern Generators
Vocalization
Animal
Neuroscience
030217 neurology & neurosurgery
Zdroj: The Journal of Comparative Neurology
ISSN: 1096-9861
Popis: Vocalization is a behavioral feature that is shared among multiple vertebrate lineages, including fish. The temporal patterning of vocal communication signals is set, in part, by central pattern generators (CPGs). Toadfishes are well‐established models for CPG coding of vocalization at the hindbrain level. The vocal CPG comprises three topographically separate nuclei: pre‐pacemaker, pacemaker, motor. While the connectivity between these nuclei is well understood, their neurochemical profile remains largely unexplored. The highly vocal Gulf toadfish, Opsanus beta, has been the subject of previous behavioral, neuroanatomical and neurophysiological studies. Combining transneuronal neurobiotin‐labeling with immunohistochemistry, we map the distribution of inhibitory neurotransmitters and neuromodulators along with gap junctions in the vocal CPG of this species. Dense GABAergic and glycinergic label is found throughout the CPG, with labeled somata immediately adjacent to or within CPG nuclei, including a distinct subset of pacemaker neurons co‐labeled with neurobiotin and glycine. Neurobiotin‐labeled motor and pacemaker neurons are densely co‐labeled with the gap junction protein connexin 35/36, supporting the hypothesis that transneuronal neurobiotin‐labeling occurs, at least in part, via gap junction coupling. Serotonergic and catecholaminergic label is also robust within the entire vocal CPG, with additional cholinergic label in pacemaker and prepacemaker nuclei. Likely sources of these putative modulatory inputs are neurons within or immediately adjacent to vocal CPG neurons. Together with prior neurophysiological investigations, the results reveal potential mechanisms for generating multiple classes of social context‐dependent vocalizations with widely divergent temporal and spectral properties.
Databáze: OpenAIRE
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