Decreased locomotor activity in mice expressing tTA under control of the CaMKIIα promoter
| Autor: | J. L. Lowing, G. L. Schafer, Audrey F. Seasholtz, J. S. Schneider, S. Mohan, Brandon C. McKinney, Geoffrey G. Murphy, Huda Akil, Roger L. Albin, M. X. Zhao, M. Y. Heng |
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| Rok vydání: | 2008 |
| Předmět: |
Proteomics
Genetically modified mouse medicine.medical_specialty Light Transgene Morris water navigation task Mice Transgenic Anxiety Motor Activity Biology Mice Behavioral Neuroscience Transactivation Internal medicine Genetics medicine Animals Fear conditioning Maze Learning Promoter Regions Genetic Kinase Darkness Tetracycline Phenotype Motor coordination Mice Inbred C57BL Endocrinology Neurology Immunology Exploratory Behavior Trans-Activators Calcium-Calmodulin-Dependent Protein Kinase Type 2 Protein Processing Post-Translational |
| Zdroj: | Genes, Brain and Behavior. 7:203-213 |
| ISSN: | 1601-183X 1601-1848 |
| DOI: | 10.1111/j.1601-183x.2007.00339.x |
| Popis: | Transgenic mice in which the tetracycline transactivator (tTA) is driven by the forebrain-specific calcium-calmodulin-dependent kinase II alpha promoter (CaMKII alpha-tTA mice) are used to study the molecular genetics of many behaviors. These mice can be crossed with other transgenic mice carrying a transgene of interest coupled to the tetracycline-responsive promoter element to produce mice with forebrain-specific expression of the transgene under investigation. The value of using CaMKII alpha-tTA mice to study behavior, however, is dependent on the CaMKII alpha-tTA mice themselves lacking a behavioral phenotype with respect to the behaviors being studied. Here we present data that suggest CaMKII alpha-tTA mice have a behavioral phenotype distinct from that of their wild-type (WT) littermates. Most strikingly, we find that CaMKII alpha-tTA mice, both those with a C57BL/6NTac genetic background (B6-tTA) and those with a 129S6B6F1/Tac hybrid genetic background (F1-tTA), exhibit decreased locomotor activity compared with WT littermates that could be misinterpreted as altered anxiety-like behavior. Despite this impairment, neither B6-tTA nor F1-tTA mice perform differently than their WT littermates in two commonly used learning and memory paradigms - Pavlovian fear conditioning and Morris water maze. Additionally, we find data regarding motor coordination and balance to be mixed: B6-tTA mice, but not F1-tTA mice, exhibit impaired performance on the accelerating rotarod and both perform as well as their WT littermates on the balance beam. |
| Databáze: | OpenAIRE |
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