Purification of a lectin from Arisaema erubescens (Wall.) Schott and its pro-inflammatory effects

Autor: Hong Li Yu, Xian Qiong Liu, Yao Zong Pan, Hao Wu, Teng Fei Zhao, Run Jun Shi
Rok vydání: 2011
Předmět:
Male
Erythrocytes
paw edema
Arisaema erubescens
Neutrophils
medicine.medical_treatment
Arisaema erubescens lectin (AEL)
Pharmaceutical Science
High-performance liquid chromatography
Plant Roots
Analytical Chemistry
Rats
Sprague-Dawley
Cell Movement
pro-inflammatory compounds
Drug Discovery
Edema
Mast Cells
Fetuins
Polyacrylamide gel electrophoresis
education.field_of_study
biology
Chemistry
Mast cell
medicine.anatomical_structure
Chemistry (miscellaneous)
Molecular Medicine
Rabbits
Metacarpus
Plant Lectins
Prostaglandin E
Population
Asialoglycoproteins
Nitric Oxide
Dinoprostone
Article
lcsh:QD241-441
lcsh:Organic chemistry
medicine
Animals
p-Methoxy-N-methylphenethylamine
Physical and Theoretical Chemistry
education
Chromatography
Tumor Necrosis Factor-alpha
Peritoneal fluid
Hemagglutination
Organic Chemistry
Lectin
biology.organism_classification
Molecular biology
Arisaema
Rats
haemagglutination activity
biology.protein
Macrophages
Peritoneal
neutrophil migration
Zdroj: Molecules
Volume 16
Issue 11
Pages: 9480-9494
Molecules, Vol 16, Iss 11, Pp 9480-9494 (2011)
ISSN: 1420-3049
Popis: The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 μg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 μg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 μg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E(2 )(PGE(2)) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the number of resident cells following peritoneal lavage did not reduce the AEL-induced neutrophil migration. However, pre-treatment with 3% thioglycollate which increased the peritoneal macrophage population by 201%, enhanced the neutrophil migration induced by AEL (200 μg/mL/cavity) (p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of rat peritoneal cavities with compound 48/80 (N-methyl-p-methoxyphenethylamine with formaldehyde) did not modify AEL-induced neutrophil migration. The results provided the basis for identifying the toxic components of A. erubescens and AEL could be a new useful tool for pro-inflammatory research.
Databáze: OpenAIRE
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