MicroRNAs in Hyperglycemia Induced Endothelial Cell Dysfunction

Autor: Dwi Setyowati Karolina, Maskomani Silambarasan, Kandiah Jeyaseelan, Jun Rong Tan, Arunmozhiarasi Armugam, Charanjit Kaur
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
medicine.medical_specialty
Cell Survival
Biology
Catalysis
Umbilical vein
Article
endothelial dysfunction
Inorganic Chemistry
Diabetic nephropathy
lcsh:Chemistry
03 medical and health sciences
Internal medicine
Diabetes mellitus
medicine
Human Umbilical Vein Endothelial Cells
Animals
Humans
Prediabetes
Physical and Theoretical Chemistry
Endothelial dysfunction
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
microRNA
Vascular disease
Gene Expression Profiling
Organic Chemistry
apoptosis
Type 2 Diabetes Mellitus
Endothelial Cells
General Medicine
medicine.disease
diabetes mellitus
hyperglycemia
Computer Science Applications
Rats
Endothelial stem cell
MicroRNAs
030104 developmental biology
Endocrinology
Glucose
lcsh:Biology (General)
lcsh:QD1-999
Diabetes Mellitus
Type 2
Caspases
Hyperglycemia
Zdroj: International Journal of Molecular Sciences; Volume 17; Issue 4; Pages: 518
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 17, Iss 4, p 518 (2016)
ISSN: 1422-0067
DOI: 10.3390/ijms17040518
Popis: Hyperglycemia is closely associated with prediabetes and Type 2 Diabetes Mellitus. Hyperglycemia increases the risk of vascular complications such as diabetic retinopathy, diabetic nephropathy, peripheral vascular disease and cerebro/cardiovascular diseases. Under hyperglycemic conditions, the endothelial cells become dysfunctional. In this study, we investigated the miRNA expression changes in human umbilical vein endothelial cells exposed to different glucose concentrations (5, 10, 25 and 40 mM glucose) and at various time intervals (6, 12, 24 and 48 h). miRNA microarray analyses showed that there is a correlation between hyperglycemia induced endothelial dysfunction and miRNA expression. In silico pathways analyses on the altered miRNA expression showed that the majority of the affected biological pathways appeared to be associated to endothelial cell dysfunction and apoptosis. We found the expression of ten miRNAs (miR-26a-5p, -26b-5p, 29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -140-5p, -192-5p, -221-3p and -320a) to increase gradually with increasing concentration of glucose. These miRNAs were also found to be involved in endothelial dysfunction. At least seven of them, miR-29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -221-3p, -320a and -192-5p, can be correlated to endothelial cell apoptosis.
Databáze: OpenAIRE
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