Microflow Cytometers with Integrated Hydrodynamic Focusing
| Autor: | Janko Theisen, Marcin Frankowski, Andreas Kummrow, Peter Simon, Martin Schmidt, Hülya Ragusch, Nicole Bock, Jörg Neukammer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
CD3 Complex Optical Phenomena Flow (psychology) Microfluidics Analytical chemistry single cell analysis lcsh:Chemical technology Biochemistry Article Fluorescence Analytical Chemistry law.invention hydrodynamic focusing blood cell differentiation law Humans lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Fluorescent Dyes Blood Cells lab-on-a-chip Chemistry flow cytometry ultraprecision milling Phycoerythrin Mechanics Lab-on-a-chip microfluidics Atomic and Molecular Physics and Optics Vortex Volumetric flow rate Cascade Calibration Hydrodynamic focusing Hydrodynamics Particle Rheology Fluorescein-5-isothiocyanate |
| Zdroj: | Sensors (Basel, Switzerland) Sensors, Vol 13, Iss 4, Pp 4674-4693 (2013) Sensors; Volume 13; Issue 4; Pages: 4674-4693 |
| ISSN: | 1424-8220 |
| Popis: | This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample--A suspension of micro particles or blood cells--is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|