Lipidomics for Clinical Diagnosis: Dye-Assisted Laser Desorption/Ionization (DALDI) Method for Lipids Detection in MALDI Mass Spectrometry Imaging
| Autor: | Olivier Kerdraon, Isabelle Fournier, Rémi Longuespée, Karim Arafah, Michel Salzet, Annie Desmons |
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| Rok vydání: | 2014 |
| Předmět: |
Tissue Fixation
Population Mass spectrometry Tandem mass spectrometry Biochemistry Mass spectrometry imaging Matrix (chemical analysis) Tandem Mass Spectrometry Oxazines Lipidomics Biomarkers Tumor Genetics Animals Humans Coloring Agents education Molecular Biology Ovarian Neoplasms education.field_of_study Chromatography Staining and Labeling Chemistry Brain Lipid metabolism Lipid Metabolism Lipids Rats Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Medicine Female Azo Compounds Biotechnology |
| Zdroj: | OMICS: A Journal of Integrative Biology. 18:487-498 |
| ISSN: | 1557-8100 1536-2310 |
| DOI: | 10.1089/omi.2013.0175 |
| Popis: | Lipid-based biomarkers for research and diagnosis are rapidly emerging to unpack the basis of person-to-person and population variations in disease susceptibility, drug and nutritional responses, to name but a few. Hence, with the advent of MALDI Mass Spectrometry Imaging, lipids have begun to be investigated intensively. However, lipids are highly mobile during tissue preparation, and are soluble in the solvent used for matrix preparation or in the fixing fluid such as formalin, resulting in substantial delocalization. In the present article, we investigated as another alternative, the possibility of using specific dyes that can absorb UV wavelengths, in order to desorb the lipids specifically from tissue sections, and are known to immobilize them in tissues. Indeed, after lipid insolubilization with chromate solution or chemical fixation with osmium tetroxide, heterocyclic-based dyes can be directly used without matrix. Taking into account the fact that some dyes have this matrix-free capability, we identified particular dyes dedicated to histological staining of lipids that could be used with MALDI mass spectrometry imaging. We stained tissue sections with either Sudan Black B, Nile Blue A, or Oil Red O. An important advantage of this assay relies on its compatibility with usual practices of histopathological investigation of lipids. As a new method, DALDI stands for Dye-Assisted Laser Desorption Ionization and allows for future clinical and histopathological applications using routine histological protocols. Additionally, this novel methodology was validated in human ovarian cancer biopsies to demonstrate its use as a suitable procedure, for histological diagnosis in lipidomics field. |
| Databáze: | OpenAIRE |
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