Extending the Genotype inBrachypodiumby Including DNA Methylation Reveals a Joint Contribution with Genetics on Adaptive Traits
| Autor: | Jared Streich, Adam J Reddiex, Justin O. Borevitz, Alison Heussler, Akanksha Srivastava, Diep Ganguly, Pip B Wilson, Steven R. Eichten |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
brachypodium QH426-470 Quantitative trait locus 01 natural sciences chemistry.chemical_compound 03 medical and health sciences Missing heritability problem Genetic variation Genotype Genetics Epigenetics Molecular Biology Genetics (clinical) 030304 developmental biology Epigenomics 0303 health sciences Genetic diversity biology biology.organism_classification Phenotype chemistry epigenomics missing heritability DNA methylation Brachypodium DNA 010606 plant biology & botany |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 10, Iss 5, Pp 1629-1637 (2020) |
| ISSN: | 2160-1836 |
| Popis: | Epigenomic changes have been considered a potential missing link underlying phenotypic variation in quantitative traits but is potentially confounded with the underlying DNA sequence variation. Although the concept of epigenetic inheritance has been discussed in depth, there have been few studies attempting to directly dissect the amount of epigenomic variation within inbred natural populations while also accounting for genetic diversity. By using known genetic relationships betweenBrachypodiumlines, multiple sets of nearly identical accession families were selected for phenotypic studies and DNA methylome profiling to investigate the dual role of (epi)genetics under simulated natural seasonal climate conditions. Despite reduced genetic diversity, appreciable phenotypic variation was still observable in the measured traits (height, leaf width and length, tiller count, flowering time, ear count) between as well as within the inbred accessions. However, with reduced genetic diversity there was diminished variation in DNA methylation within families. Mixed-effects linear modelling revealed large genetic differences between families and a minor contribution of epigenomic variation on phenotypic variation in select traits. Taken together, this analysis suggests a limited but significant contribution of DNA methylation towards heritable phenotypic variation relative to genetic differences. |
| Databáze: | OpenAIRE |
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