| Autor: |
Meng Jiang, Tianbao Dong, Chaowei Han, Luyao Liu, Tiantian Zhang, Qing Kang, Pengcheng Wang, Feimeng Zhou |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 1208:339830 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/j.aca.2022.339830 |
| Popis: |
Current serological antibody tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) require enzyme or fluorescent labels, and the titer well plates cannot be reused. By immobilizing histidine (His)-tagged SARS-CoV-2 spike (S1) protein onto tris‒nitrilotriacetic acid (tris-NTA) sensor and using the early association phase for mass-transfer-controlled concentration determination, we developed a rapid and regenerable surface plasmon resonance (SPR) method for quantifying anti-SARS-CoV-2 antibody. On a five-channel SPR instrument and with optimized S1 protein immobilization density, each of the four analytical channels is sequentially used for multiple measurements, and all four channels can be simultaneously regenerated once they have reached a threshold value. Coupled with a programmable autosampler, each sensor can be regenerated at least 20 times, enabling uninterrupted assays of more than 800 serum samples. The accuracy and speed of our method compare well with those of the enzyme-linked immunosorbent assay (ELISA), and the detection limit (0.057 μg mL |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect