Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients
| Autor: | Aleksandar J. Ristić, Vid Simon Šelih, Miloš Opačić, Slobodan Savic, Ivan Spasojevic, Dragoslav Sokić, Danijela Savic, Vladimir Baščarević, Maja Zorović, Marko Živin, Savo Raičević |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty chemistry.chemical_element LA‐ICP‐MS Mitochondrion lcsh:RC321-571 03 medical and health sciences Behavioral Neuroscience Epilepsy 0302 clinical medicine cytochrome c oxidase medicine Metalloprotein Cytochrome c oxidase lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry 030304 developmental biology Original Research chemistry.chemical_classification 0303 health sciences Hippocampal sclerosis biology Chemistry temporal lobe epilepsy medicine.disease Electron transport chain Copper nervous system diseases medicine.anatomical_structure nervous system copper hippocampal sclerosis biology.protein Neuron 030217 neurology & neurosurgery |
| Zdroj: | Brain and Behavior Brain and Behavior, Vol 11, Iss 2, Pp n/a-n/a (2021) |
| ISSN: | 2162-3279 |
| Popis: | Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper‐containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE‐HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery. Regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase in coronal sections of sclerotic hippocampi of epilepsy patients and controls. |
| Databáze: | OpenAIRE |
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