Additional file 8 of Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation

Autor: Dietrich, Mariola A., Mikołaj Adamek, Jung-Schroers, Verena, Rakus, Krzysztof, Chadzińska, Magdalena, Hejmej, Anna, Hliwa, Piotr, Bilińska, Barbara, Karol, Halina, Ciereszko, Andrzej
Rok vydání: 2020
DOI: 10.6084/m9.figshare.13290937
Popis: Additional file 8: Isolation of Wap65-2 using hemin-agarose affinity chromatography. SP-carp seminal plasma, 1-4 unbound fractions eluted with binding buffer (10 mM sodium phosphate, pH 7.4; 0.5 M NaCl), 5–9 bound fractions containing Wap65-2 eluted with elution buffer 0.2 M sodium citrate, pH 5.2; 0.5 M NaCl and 0.02% NaN3).
Databáze: OpenAIRE