opl: a zinc finger protein that regulates neural determination and patterning in Xenopus
| Autor: | Vladimir Apekin, Xuedong Liu, Christa Merzdorf, John S. Kuo, Hazel Sive, Mukesh Patel, Joshua T. Gamse |
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| Rok vydání: | 1998 |
| Předmět: |
animal structures
Molecular Sequence Data Ectoderm Xenopus Proteins Biology Models Biological Nervous System ZIC1 Xenopus laevis Cell Movement medicine Animals Paired Box Transcription Factors Amino Acid Sequence Noggin PAX3 Transcription Factor Molecular Biology Body Patterning Embryonic Induction Neural fold Sequence Homology Amino Acid Neural tube Proteins Neural crest Cell Differentiation Zinc Fingers Antigens Differentiation Molecular biology DNA-Binding Proteins stomatognathic diseases medicine.anatomical_structure Neurula Neural Crest embryonic structures Trans-Activators Snail Family Transcription Factors Epidermis Carrier Proteins Neural plate Transcription Factors Developmental Biology |
| Zdroj: | Europe PubMed Central Scopus-Elsevier |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.125.15.2867 |
| Popis: | In order to study the mechanism of neural patterning in Xenopus, we used subtractive cloning to isolate genes activated early during this process. One gene isolated was opl, (odd-paired-like) that resembles the Drosophila pair-rule gene odd-paired and encodes a zinc finger protein that is a member of the Zic gene family. At the onset of gastrulation, opl is expressed throughout the presumptive neural plate, indicating that neural determination has begun at this stage while, by neurula, opl expression is restricted to the dorsal neural tube and neural crest. opl encodes a transcriptional activator, with a carboxy terminal regulatory domain, which when removed increases opl activity. opl both sensitizes animal cap ectoderm to the neural inducer noggin and alters the spectrum of genes induced by noggin, allowing activation of the midbrain marker engrailed. Consistent with the later dorsal neural expression of opl, the activated form of opl is able to induce neural crest and dorsal neural tube markers both in animal caps and whole embryos. In ventral ectoderm, opl induces formation of loose cell aggregates that may indicate neural crest precursor cells. Aggregates do not express an epidermal marker, indicating that opl suppresses ventral fates. Together, these data suggest that opl may mediate neural competence and may be involved in activation of midbrain, dorsal neural and neural crest fates. |
| Databáze: | OpenAIRE |
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