Downregulation by a long-acting beta2-adrenergic receptor agonist and corticosteroid of Staphylococcus aureus-induced airway epithelial inflammatory mediator production
| Autor: | Malcolm Johnson, Edith Puchelle, C. Kileztky, Chantal Trentesaux, Odile Bajolet, Konstantina Fragaki, Jean-Marie Zahm |
|---|---|
| Přispěvatelé: | Dynamique cellulaire et moléculaire de la muqueuse respiratoire, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Reims Champagne-Ardenne (URCA), GlaxoSmithKline (GSK), Research and Development, Birembaut, Philippe |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Physiology
Gene Expression MESH: Adrenergic beta-Agonists [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract MESH: Down-Regulation 0302 clinical medicine Receptor Extracellular Signal-Regulated MAP Kinases MESH: Extracellular Signal-Regulated MAP Kinases Cell Line Transformed 0303 health sciences MESH: Cytokines Virulence NF-kappa B Interleukin Adrenergic beta-Agonists Fluticasone-Salmeterol Drug Combination 3. Good health I-kappa B Kinase Trachea Drug Combinations Cytokines Tumor necrosis factor alpha medicine.symptom Inflammation Mediators Glucocorticoid medicine.drug Pulmonary and Respiratory Medicine Agonist medicine.medical_specialty Staphylococcus aureus MESH: Gene Expression medicine.drug_class Virulence Factors MESH: Inflammation Mediators Down-Regulation Inflammation Respiratory Mucosa Biology Proinflammatory cytokine 03 medical and health sciences Downregulation and upregulation Physiology (medical) Internal medicine MESH: JNK medicine Humans Albuterol MESH: I-kappa B Kinase MESH: Cell Line Transformed Glucocorticoids 030304 developmental biology MESH: Drug Combinations MESH: Humans MESH: Albuterol JNK Mitogen-Activated Protein Kinases Cell Biology Pneumonia Androstadienes Transcription Factor AP-1 Endocrinology 030228 respiratory system Solubility MESH: Androstadienes [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract MESH: Glucocorticoids |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology American Journal of Physiology-Lung Cellular and Molecular Physiology, American Physiological Society, 2006, 291 (1), pp.L11-8. ⟨10.1152/ajplung.00488.2005⟩ |
| ISSN: | 1040-0605 1522-1504 |
| Popis: | Although Staphylococcus aureus is a major cause of pulmonary infection, the role played by this bacterium in the induction of inflammation of human airway epithelial cells (HAEC) is poorly understood. In this study, we investigated the inflammatory response of HAEC to S. aureus soluble virulence factors and demonstrate that the combination of a long-acting β2-adrenergic receptor agonist (salmeterol) with a glucocorticoid (fluticasone propionate) has an anti-inflammatory effect on HAEC. First, we demonstrate increased expression at both the mRNA and protein levels of interleukin (IL)-8, IL-6, and tumor necrosis factor (TNF)-α following incubation of HAEC in the presence of S. aureus soluble virulence factors and the increase of 1) the free nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) activities and 2) the phosphorylated (P-) extracellular signal-regulated kinases 1 and 2 (ERK1/ERK2), the P-c-Jun NH2-terminal kinase (JNK), and the P-isoform-α of the NF-κB inhibitor (IκBα). Next, when HAEC were preincubated with the combination of salmeterol and fluticasone propionate, the inflammatory response of HAEC was markedly attenuated in that levels of IL-8, IL-6, TNF-α, NF-κB, AP-1, P-ERK1/ERK2, P-JNK, and P-IκBα decreased significantly. These data emphasize the deleterious effect of S. aureus soluble virulence factors and suggest that the combination of a β2-adrenergic receptor agonist with a glucocorticoid may attenuate the associated airway epithelial inflammation. |
| Databáze: | OpenAIRE |
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