Evidence of closely related picobirnavirus strains circulating in humans and pigs in Argentina
Autor: | Silvia Nates, Mildre C. Valle, Gisela Masachessi, Mar La B. Isa, Miguel O. Giordano, Laura C. Martinez, Pablo U. Massari, Patricia A. Barril, Leonardo J. Ferreyra |
---|---|
Rok vydání: | 2011 |
Předmět: |
Diarrhea
Microbiology (medical) Swine Molecular Sequence Data Argentina Picobirnavirus Host Specificity RNA Virus Infections Phylogenetics Genomic Segment Animals Humans Clade Phylogeny Feces Genetics Base Sequence biology Phylogenetic tree Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis RNA Host (biology) Nucleotide Mapping Amplicon RNA-Dependent RNA Polymerase biology.organism_classification Infectious Diseases |
Zdroj: | Journal of Infection. 62:45-51 |
ISSN: | 0163-4453 |
Popis: | Summary Background On the basis of the published literature, it is still difficult to draw conclusions as to whether picobirnavirus (PBV) circulation is influenced by host species restriction. Objective To provide data regarding the genetic relatedness between porcine and human PBV strains present in Argentina as a means of defining the host range and epidemiology of these viruses. Methods Fecal specimens ( n = 74) collected from kidney transplant patients ( n = 55) and piglets ( n = 19) were analyzed by RT-PCR using primers designed to amplify the porcine PBV genomic segment 2. Amplified sequences were further examined phylogenetically. Results By RT-PCR amplification 14 of 74 samples rendered amplicons of the expected 282 base pair size (8 detected from humans and 6 from pigs). Eleven amplicons (5 from humans and 6 from pigs) were selected for sequencing and subjected to phylogenetic analysis. The eleven amplicons revealed similarities between human and porcine viral sequences that ranged between 94.7 and 100% in identity. Phylogenetic analysis identified these 11 strains as PBV genogroup I-related strains and showed that they grouped as a single separate clade distinct from other PBV strains detected in humans and porcine from other countries. Conclusions The present study suggests that closely related PBV strains infect both pigs and humans in Argentina and that the epidemiology of PBVs is not species restricted. |
Databáze: | OpenAIRE |
Externí odkaz: |