Local Chemical Stimulation of Neurons with the Fluidic Force Microscope (FluidFM)
| Autor: | Doris Ling, Janos Vörös, Moritz Schneider, Csaba Forró, José F. Saenz Cogollo, Mathias J. Aebersold, Hana Han, Conrad Burchert, Shiang-Chi Lin, László Demkó, Harald Dermutz, Tomaso Zambelli |
|---|---|
| Přispěvatelé: | University of Zurich, Vörös, János |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microscope 610 Medicine & health Nanotechnology Stimulation 3107 Atomic and Molecular Physics and Optics Hippocampal formation Microscopy Atomic Force law.invention 170 Ethics 03 medical and health sciences 0302 clinical medicine law Atomic and Molecular Physics Biological neural network Animals 10237 Institute of Biomedical Engineering Fluidics Rats Wistar Physical and Theoretical Chemistry Receptor Electrodes Cells Cultured Neurons Chemistry Glutamate receptor Multielectrode array Stimulation Chemical Atomic and Molecular Physics and Optics Rats 030104 developmental biology Female and Optics 1606 Physical and Theoretical Chemistry Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | ChemPhysChem. 19:1234-1244 |
| ISSN: | 1439-4235 |
| DOI: | 10.1002/cphc.201700780 |
| Popis: | Physiological communication between neurons is dependent on the exchange of neurotransmitters at the synapses. Although this chemical signal transmission targets specific receptors and allows for subtle adaptation of the action potential, in vitro neuroscience typically relies on electrical currents and potentials to stimulate neurons. The electric stimulus is unspecific and the confinement of the stimuli within the media is technically difficult to control and introduces large artifacts in electric recordings of the activity. Here, we present a local chemical stimulation platform that resembles in vivo physiological conditions and can be used to target specific receptors of synapses. Neurotransmitters were dispensed using the force-controlled fluidic force microscope (FluidFM) nanopipette, which provides exact positioning and precise liquid delivery. We show that controlled release of the excitatory neurotransmitter glutamate induces spiking activity in primary rat hippocampal neurons, as measured by concurrent electrical and optical recordings using a microelectrode array and a calcium-sensitive dye, respectively. Furthermore, we characterized the glutamate dose response of neurons by applying stimulation pulses of glutamate with concentrations from 0 to 0.5 mm. This new stimulation approach, which combines FluidFM for gentle and precise positioning with a microelectrode array read-out, makes it possible to modulate the activity of individual neurons chemically and simultaneously record their induced activity across the entire neuronal network. The presented platform not only offers a more physiological alternative compared with electrical stimulation, but also provides the possibility to study the effects of the local application of neuromodulators and other drugs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text