Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells
| Autor: | T. N. Dugina, E. E. Balashova, N. V. Kabaeva, N E Volgina, Yu. A. Romanov, Gennady T. Sukhikh |
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| Rok vydání: | 2017 |
| Předmět: |
Serum
0301 basic medicine Cellular differentiation Cell Culture Techniques Biology Umbilical cord General Biochemistry Genetics and Molecular Biology Flow cytometry Andrology 03 medical and health sciences Blood serum Antigens CD Adipocytes medicine Animals Humans Cells Cultured Cell Proliferation Osteoblasts medicine.diagnostic_test Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Fetal Blood Flow Cytometry Cord lining Culture Media 030104 developmental biology medicine.anatomical_structure Cell culture Cattle Biomarkers Fetal bovine serum |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 162:528-533 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-017-3654-9 |
| Popis: | Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes. |
| Databáze: | OpenAIRE |
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