High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease
| Autor: | Brian P. Leung, Felix J. Hartmann, Jonathan Kipnis, Dunja Mrdjen, Bettina Schreiner, Anto Pavlovic, Iva Lelios, Burkhard Becher, Doron Merkler, Sebastian G. Utz, Melanie Greter, Frank L. Heppner |
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| Přispěvatelé: | University of Zurich, Becher, Burkhard |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Central Nervous System Aging Myeloid Immunology Central nervous system 610 Medicine & health Disease High dimensional Biology ddc:616.07 10263 Institute of Experimental Immunology 03 medical and health sciences Mice Immune system Immunity medicine Leukocytes Immunology and Allergy Animals Mass cytometry Tissue homeostasis UFSP13-5 Translational Cancer Research 2403 Immunology Microglia Multiple sclerosis Macrophages Experimental autoimmune encephalomyelitis Cell mapping Neurodegenerative Diseases Dendritic Cells 2725 Infectious Diseases medicine.disease 10040 Clinic for Neurology Mice Inbred C57BL 030104 developmental biology Infectious Diseases medicine.anatomical_structure 2723 Immunology and Allergy 570 Life sciences biology Single-Cell Analysis Neuroscience |
| Zdroj: | Immunity, Vol. 48, No 2 (2018) pp. 380-395.e6 |
| ISSN: | 1074-7613 |
| Popis: | Individual reports suggest that the central nervous system (CNS) contains multiple immune cell types with diverse roles in tissue homeostasis, immune defense, and neurological diseases. It has been challenging to map leukocytes across the entire brain, and in particular in pathology, where phenotypic changes and influx of blood-derived cells prevent a clear distinction between reactive leukocyte populations. Here, we applied high-dimensional single-cell mass and fluorescence cytometry, in parallel with genetic fate mapping systems, to identify, locate, and characterize multiple distinct immune populations within the mammalian CNS. Using this approach, we revealed that microglia, several subsets of border-associated macrophages and dendritic cells coexist in the CNS at steady state and exhibit disease-specific transformations in the immune microenvironment during aging and in models of Alzheimer's disease and multiple sclerosis. Together, these data and the described framework provide a resource for the study of disease mechanisms, potential biomarkers, and therapeutic targets in CNS disease. |
| Databáze: | OpenAIRE |
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