Fibroblasts isolated from a keratocystic odontogenic tumor promote osteoclastogenesis in vitro via interaction with epithelial cells
| Autor: | Li Tj, Sima Zh, Wei-Peng Jiang, Hong Wang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Gingiva
Osteoclasts Odontogenic Tumors Cell Communication Bone resorption Mice Osteoclast Precursor cell medicine Animals Humans Bone Resorption Fibroblast General Dentistry Osteoblasts biology Chemistry RANK Ligand Cell Differentiation Epithelial Cells Fibroblasts Epithelium In vitro Coculture Techniques Up-Regulation medicine.anatomical_structure RAW 264.7 Cells Otorhinolaryngology RANKL Immunology Cancer research biology.protein Keratocystic Odontogenic Tumor |
| Zdroj: | Oral diseases. 21(2) |
| ISSN: | 1601-0825 |
| Popis: | Objectives Investigate the role of the epithelial–mesenchymal interaction of keratocystic odontogenic tumor (KCOT) in influencing osteoclastogenesis. Materials and Methods Fibroblasts isolated from KCOT fibrous capsule and normal gingival mucosa were, respectively, co-cultured with human immortalized oral epithelial cells (HIOECs), and the supernatant was collected to make conditioned medium, in which the osteoclastogenesis of osteoclast precursor cell line Raw 264.7 was observed. Genes related to bone resorption (RANKL, OPG, COX-2, and M-CSF) were analyzed by real-time PCR. Antibodies against human sRANKL and inhibitor of COX-2: NS398 were added to conditioned medium to investigate the inhibitory effect on osteoclastogenesis. Results Compared with co-cultured gingival fibroblasts and HIOECs (GE-CM), the conditioned medium from co-cultured KCOT fibroblasts and HIOECs (KE-CM) induced more osteoclast-like cell formation and increased NFATC1 mRNA in Raw264.7 cells (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text