Improved determination of the bisphosphonate pamidronate disodium in plasma and urine by pre-column derivatization with fluorescamine, high-performance liquid chromatography and fluorescence detection
| Autor: | G. Flesch, N. Tominaga, P.H. Degen |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Detection limit
Chromatography Diphosphonates Pamidronate Hydrochloric acid Ethylenediaminetetraacetic acid General Chemistry Urine Fluorescamine High-performance liquid chromatography Fluorescence Fluorescence spectroscopy chemistry.chemical_compound chemistry Humans Infusions Intravenous Derivatization Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 568:261-266 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(91)80362-g |
| Popis: | An improved method for the determination of 1-hydroxy-3-aminopropylidene-1,1-bisphosphonate (pamidronate) in human urine and plasma is described. The procedure is based on a co-precipitation of the bisphosphonates (pamidronate and 6-amino-1-hydroxypentilidene-bisphosphonate, used as internal standard) with calcium phosphate. After centrifugation the precipitate is redissolved in hydrochloric acid, followed by a second precipitation. Then the bisphosphonates are dissolved in ethylenediaminetetraacetic acid, derivatized with fluorescamine, and separated by high-performance liquid chromatography. Using fluorescence detection, the limit of quantitation for pamidronate was 0.8 mumol/l in plasma and 0.7 mumol/l in urine. |
| Databáze: | OpenAIRE |
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