Acid-sensing ion channels are involved in epithelial Na+uptake in the rainbow troutOncorhynchus mykiss
| Autor: | Salvatore D. Blair, Agnieszka K. Dymowska, Danuta Chamot, Greg G. Goss, Aaron G. Schultz |
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| Rok vydání: | 2014 |
| Předmět: |
Gills
Gill endocrine system animal structures Indoles Physiology animal diseases Sodium Sodium-Potassium-Exchanging ATPase Biological Transport Active chemistry.chemical_element Biology digestive system Antibodies Amiloride medicine Animals Amino Acid Sequence Cloning Molecular Ion channel Acid-sensing ion channel urogenital system Articles Cell Biology Acid Sensing Ion Channel Blockers Trypanocidal Agents Cell biology Acid Sensing Ion Channels chemistry Biochemistry Oncorhynchus mykiss Rainbow trout Diminazene Sequence Alignment medicine.drug |
| Zdroj: | American Journal of Physiology-Cell Physiology. 307:C255-C265 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00398.2013 |
| Popis: | A role for acid-sensing ion channels (ASICs) to serve as epithelial channels for Na+uptake by the gill of freshwater rainbow trout was investigated. We found that the ASIC inhibitors 4′,6-diamidino-2-phenylindole and diminazene decreased Na+uptake in adult rainbow trout in a dose-dependent manner, with IC50values of 0.12 and 0.96 μM, respectively. Furthermore, we cloned the trout ASIC1 and ASIC4 homologs and demonstrated that they are expressed differentially in the tissues of the rainbow trout, including gills and isolated mitochondrion-rich cells. Immunohistochemical analysis using custom-made anti-zASIC4.2 antibody and the Na+-K+-ATPase (α5-subunit) antibody demonstrated that the trout ASIC localizes to Na+/K+-ATPase-rich cells in the gill. Moreover, three-dimensional rendering of confocal micrographs demonstrated that ASIC is found in the apical region of mitochondrion-rich cells. We present a revised model whereby ASIC4 is proposed as one mechanism for Na+uptake from dilute freshwater in the gill of rainbow trout. |
| Databáze: | OpenAIRE |
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