Glutamate uptake in cultured astrocytes depends on age: a study about the effect of guanosine and the sensitivity to oxidative stress induced by H2O2
| Autor: | Francine Tramontina, Daniela Aparecida de Godoi Gonçalves, Renato Dutra Dias, Carmem Gottfried, Emílio Hideyuki Moriguchi, Diogo O. Souza, Carlos A. Gonçalves, Susana Tchernin Wofchuk |
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| Rok vydání: | 2002 |
| Předmět: |
Aging
medicine.medical_specialty Excitotoxicity Glutamic Acid Guanosine S100 Calcium Binding Protein beta Subunit medicine.disease_cause chemistry.chemical_compound Internal medicine Glial Fibrillary Acidic Protein medicine Animals Nerve Growth Factors Rats Wistar Neurotransmitter Cells Cultured Cerebral Cortex Glial fibrillary acidic protein biology S100 Proteins Glutamate receptor Hydrogen Peroxide Rats Oxidative Stress Endocrinology medicine.anatomical_structure chemistry Biochemistry Astrocytes biology.protein Neuroglia Oxidative stress Developmental Biology Astrocyte |
| Zdroj: | Mechanisms of Ageing and Development. 123:1333-1340 |
| ISSN: | 0047-6374 |
| Popis: | Relatively few studies have been conducted to investigate the relationship between glutamate and development and/or aging. Rat cortical astrocyte cultures were used as a model to investigate glutamate uptake during development. The immunocontent of the markers glial fibrillary acidic protein (GFAP) and S100B increased, while basal secretion of S100B decreased, in astrocytes from 10 to 40 days in vitro (DIV). Basal glutamate uptake increased with age. Exposure to hydrogen peroxide decreased glutamate uptake more potently at 40 than 10 DIV. Moreover, 40 DIV astrocytes showed earlier loss of integrity (at 6 h) than 10 DIV astrocytes (at 24 h) after H(2)O(2) exposure. Addition of guanosine stimulated glutamate uptake only in 10 DIV astrocytes. The present work shows that mature astrocytes in culture present some neurochemical alterations also observed in astrocytes of aged animals. These results can contribute to the understanding of some consequences of the excitotoxicity and oxidative stress during brain aging. |
| Databáze: | OpenAIRE |
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