Neurohypophyseal hormone-like peptides in the ovine pineal gland using reverse-phase liquid chromatography and radioimmunoassay
| Autor: | C. A. Salemink, Paul Pévet, H. P. J. M. Noteborn, I. Ebels, A.C. Reinharz |
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| Rok vydání: | 1988 |
| Předmět: |
endocrine system
Physiology Radioimmunoassay Peptide Vasotocin Neurophysins Peptide hormone Oxytocin Pineal Gland Biochemistry High-performance liquid chromatography Cellular and Molecular Neuroscience chemistry.chemical_compound Pineal gland Endocrinology medicine Animals Chromatography High Pressure Liquid chemistry.chemical_classification Sheep Chromatography Chemistry Arginine Vasopressin medicine.anatomical_structure nervous system Chromatography Gel Pituitary Hormones Posterior hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Peptides. 9:455-462 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/0196-9781(88)90147-7 |
| Popis: | A method is described for the determination of the neurohormone contents of ovine pineal tissue by radioimmunoassay (RIA) after successive fractionation on gel filtration in formic acid and reverse-phase liquid chromatography (HPLC). This method gives a good resolution for the neurohormones vasopressin, vasotocin and oxytocin, without a singificant interference of aspecific cross-reacting of peptides with the RIA. An acid extract from ovine pineal tissue was found to contain amounts of immunoreactive AVP- and OXT-like peptides, whereas an AVT-like peptide was not detectable over background levels after HPLC with post-column RIA. It is concluded from our results that an AVT-like peptide is not present in ovine pineal tissue, and the pineal AVP- and OXT-like peptides appeared to be associated to neurophysin molecules. |
| Databáze: | OpenAIRE |
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