Development and application of packed-column supercritical fluid chromatography/pneumatically assisted electrospray mass spectrometry
| Autor: | Timothy R. Baker, J. David Pinkston |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Packed bed
Electrospray Chromatography Structural Biology Sprayer Chemistry Supercritical fluid chromatography Analytical chemistry Atmospheric-pressure chemical ionization Direct electron ionization liquid chromatography–mass spectrometry interface Mass spectrometry High-performance liquid chromatography Spectroscopy |
| Zdroj: | Journal of the American Society for Mass Spectrometry. 9:498-509 |
| ISSN: | 1044-0305 |
| DOI: | 10.1016/s1044-0305(98)00006-3 |
| Popis: | A pneumatically assisted electrospray liquid chromatography/mass spectrometry (LC/MS) interface has been modified for use with packed-column supercritical fluid chromatography (pcSFC). The modifications include the addition of a concentric sheath-flow liquid to the spray device. This allowed the addition of modifiers at the sprayer tip that promote ionization of neutral, pcSFC-separated components. Post-column chromatographic fidelity was preserved using a novel pressure-regulation scheme. Post-column pressure regulation was accomplished by adding a “pressure-regulating fluid” (supplied under pressure control) to the effluent just ahead of the sprayer. The modified interface has been used to characterize a variety of mixtures including emollients, modified polysiloxanes, and pharmaceutical agents. The spectra produced by using this pcSFC/MS interface are similar to electrospray LC/MS spectra. |
| Databáze: | OpenAIRE |
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