Changes in the biochemical, hematological and histopathological parameters in STZ-Induced diabetic rats and the ameliorative effect of Kigelia africana fruit extract
| Autor: | O.O. Babalola, Paul O. Awoniran, Femi Kayode Agboola, Titus A.M. Msagati, Oladapo F. Fagbohun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Kigelia africana Mean corpuscular hemoglobin Pharmacology Article Glibenclamide Food science 03 medical and health sciences 0302 clinical medicine Diabetes mellitus medicine lcsh:Social sciences (General) lcsh:Science (General) Kidney Multidisciplinary medicine.diagnostic_test Mean corpuscular hemoglobin concentration business.industry Streptozotocin Health sciences food and beverages medicine.disease Metformin Chemistry Biological sciences 030104 developmental biology medicine.anatomical_structure FTIR Agricultural science Hyperglycemia lcsh:H1-99 business 030217 neurology & neurosurgery medicine.drug lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 5, Pp e03989-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Background Biochemical, hematological and histological changes are major observable clinical and pathological factors associated with Diabetes mellitus. Derangement in the levels of these parameters increases the risk of the development of complications. In another hand, gastrointestinal intolerance due to the development of lactic acidosis on the gastrointestinal tract and the intestinal microbiome is the toxic side effect of various synthetic antidiabetic agents. The use of Kigelia africana fruit extract for the treatment of diabetes has been scientifically validated. This study therefore aimed at investigating changes in the biochemical, hematological and histological parameters as well as the determination of the functional groups present in the hexane fraction of the fruit. Methods The fruits were extracted with ethanol and partitioned with n-hexane to obtain the hexane fraction. Diabetic rats induced with streptozotocin (STZ) were divided into 5 groups of 5 animals each and treated with 100, 200 and 400 mg/kg body weight (BW) hexane fraction alongside reference standard; glibenclamide. Fasting blood glucose levels and their body weights were monitored weekly. Animals were sacrificed at the end of 28-day treatment. Blood, liver, and kidney were collected for biochemical, hematological and histopathological analyses. Fourier transform infrared resonance (FTIR) spectroscopic analysis was carried out on the hexane fraction for functional group determination. Results The hexane fraction of K. africana fruit extract decreased fasting blood glucose (FBG) levels significantly with ameliorative effects on the hematological parameters such as packed cell volume (PCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and red blood cells (RBC) etc. There were significant regenerative differences in the biochemical activities as well as the renal cortex and midzone sections of the rat's kidney and liver when compared with untreated diabetic rats. The presence of polyphenolic functional groups via FTIR analysis suggested high antioxidant activities of the fruit extract. Conclusion The use of Kigelia africana fruit extracts protects against biochemical, hematological and histological changes that are injurious to diabetic patients. Therefore, Kigelia africana fruit is a good hepatic- and nephroprotective agent and has a hemato-protective ability. Diabetes mellitus; Kigelia africana; Streptozotocin; FTIR; Hyperglycemia; Metformin; Chemistry; Food Science; Agricultural Science; Biological Sciences; Health Sciences |
| Databáze: | OpenAIRE |
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