Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem Cells
| Autor: | Geun-Hui Lee, Ji-Su Kim, Young-Ho Park, Seon A Choi, Bong-Seok Song, Jong Hee Lee, Jae-Won Huh, Jae Jin Cha, Sun-Uk Kim, Bo Woong Sim, Sang-Hoon Lee, Hae Jun Yang, Sang-Rae Lee, Seung-Hwan Lee, Young-Hyun Kim, Ju-Hyun An, Yeung Bae Jin, Pil Soo Jeong |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Hematopoietic differentiation
education.field_of_study Population CD34 Retinoic acid Human induced pluripotent stem cells Cell Biology Cell fate determination Biology Regenerative medicine Cell biology Haematopoiesis chemistry.chemical_compound lineage specification chemistry Original Article Estradiol-17β Progenitor cell education Induced pluripotent stem cell Cell fate decision Developmental Biology |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 2005-3606 |
| Popis: | Background and Objectives Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during in vivo development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17-β (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. Methods and Results We used 10 nM E2, 3 µM P4, 10 nM DM, and 10 nM RA based on their functional in vivo developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34+CD45+ cells with progenitor functions, even in the CD43- population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. Conclusions Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications. |
| Databáze: | OpenAIRE |
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