Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile
| Autor: | Jochen Büchs, Martin Zimmermann, Bernd Maier, M. Silberbach |
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| Rok vydání: | 2003 |
| Předmět: |
Gluconobacter oxydans
Time Factors chemistry.chemical_element Gluconates Applied Microbiology and Biotechnology Oxygen Laboratory flask chemistry.chemical_compound Glucose dehydrogenase Chromatography Strain (chemistry) Chemistry General Medicine Carbon Dioxide Hydrogen-Ion Concentration equipment and supplies Culture Media Glucose Biochemistry Scientific method Fermentation Carbon dioxide Oxidation-Reduction Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 62:92-98 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | In this study, the advantage of a novel measuring device for the online determination of oxygen and carbon dioxide transfer rates in shaking-flasks is reported for glucose oxidation by Gluconobacter oxydans. In this fermentation process, this device was used for the characterization of the oxidation pattern of different strains. G. oxydans NCIMB 8084 forms 2,5-diketogluconate from d-glucose in a multi-stage process via three different membrane-bound dehydrogenases. This strain was chosen for a scale-up of the process from shaking-flasks to a 2-l stirred vessel. An enhancement of 2,5-diketogluconate production was realized by controlling the pH at different levels during the fermentation. |
| Databáze: | OpenAIRE |
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