In vitro labeling of proteins by reductive methylation: application to proteins involved in supramolecular structures
| Autor: | C. S. Heacock, A. S. Duhaiman, James R. Bamburg, D. A. Amorese, Barbara W. Bernstein |
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| Rok vydání: | 1982 |
| Předmět: |
Protein Conformation
Bicine Polyacrylamide macromolecular substances Tropomyosin Biochemistry Pyrophosphate Methylation chemistry.chemical_compound Animals Actinin Actin-binding protein Molecular Biology Actin Cytoskeleton biology Isoelectric focusing Sodium cyanoborohydride Proteins Cell Biology Actins chemistry biology.protein Rabbits Chickens Protein Binding |
| Zdroj: | Journal of cellular biochemistry. 19(1) |
| ISSN: | 0730-2312 |
| Popis: | Actin and tropomyosin, purified from both muscle and brain, and alpha-actinin, purified from muscle, have been labeled in vitro by reductive methylation to specific activities of greater than 10(5) dpm/micrograms protein. Actin so modified bound DNase I and polymerized identically to unmodified actin. Furthermore, the spectral properties of actin did not change after labeling. The interactions of labeled tropomyosin and alpha-actinin with F-actin were nearly identical to those of the unmodified proteins. These modified proteins comigrated with their unmodified counterparts in both SDS-containing polyacrylamide gels and isoelectric focusing gels. The labeled actin was quantitatively extracted from SDS-containing polyacrylamide gels (yield greater than 98% of radioactivity applied demonstrating that all of the radioactivity was protein bound. The reductive methylation procedure worked well at pH 8.0-8.5 in either pyrophosphate buffer or Bicine buffer using formaldehyde with [3H]-sodium borohydride as the reducing agent. The procedure could also be performed at pH 7.0 in phosphate buffer using (14C]-formaldehyde with sodium cyanoborohydride as the reducing agent. Proteins so labeled are ideal for use in quantitative experiments involving protein-protein interactions. |
| Databáze: | OpenAIRE |
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