Comparison of two encapsulation processes to protect the commensal gut probiotic bacterium Faecalibacterium prausnitzii from the digestive tract
| Autor: | Cyril Iaconelli, Sébastien Dupont, Christian Caliri, Patrick Gervais, Odile Chambin, Audrey Raise, Laurent Beney, Alexandre Charriau |
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| Přispěvatelé: | Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Procédés Microbiologiques et Biotechnologiques (PMB), Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Università degli Studi di Camerino, Physico-Chimie de l'Aliment et du Vin (PCAV), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Drug delivery system
Pharmaceutical Science Faecalibacterium prausnitzii Probiotic bacterium 02 engineering and technology ALMP beads 030226 pharmacology & pharmacy Exposure test Commensal gut probiotic bacteria Microbiology 03 medical and health sciences 0302 clinical medicine Lipid Inclusion Gut bacteria biology Chemistry 021001 nanoscience & nanotechnology biology.organism_classification [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Digestive tract Hydrophobic matrix Encapsulation 0210 nano-technology Gelucire R 43-01 Bacteria |
| Zdroj: | Journal of Drug Delivery Science and Technology Journal of Drug Delivery Science and Technology, Elsevier, 2020, 56 (Part A), pp.101608. ⟨10.1016/j.jddst.2020.101608⟩ |
| ISSN: | 1773-2247 |
| DOI: | 10.1016/j.jddst.2020.101608⟩ |
| Popis: | International audience; Anaerobic commensal gut bacteria are among the most promising future treatments for many diseases. Because of their sensitivity to oxygen and to the harsh conditions of the gut, few studies on formulations that deliver bacteria in a viable and functional form to the intestine have been done. The aim of this study was to develop a reliable and easy-to-scale-up process meeting these requirements. The designed process consisted of mixing a freeze-dried bacterium, Faecalibacterium prausnitzii, with a melted hydrophobic matrix (Gelucire® 43/01) to encapsulate them into a solid lipid base after cooling. These formulations were compared with freeze-dried beads containing bacteria encapsulated by ionotropic gelation. This work demonstrates that both formulations improve the survival of bacteria in the stomach and distal jejunum buffers during a digestive exposure test. However, only lipid inclusion provided sufficient protection suitable for therapeutic application. Nevertheless, Gelucire® poorly stabilizes bacteria during storage, unlike the freeze-dried beads. |
| Databáze: | OpenAIRE |
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