CryoEM of bacterial secretion systems: A primer for microbiologists
| Autor: | Trishant R. Umrekar, Morgan Beeby, Eli J. Cohen, Nayim Gonzalez-Rodriguez, Tina Drobnič |
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| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular 0303 health sciences Molecular model 030306 microbiology Protein Conformation Cryoelectron Microscopy Membrane Transport Proteins Computational biology Biology Microbiology Single Molecule Imaging 03 medical and health sciences Imaging Three-Dimensional Bacterial Proteins Type III Secretion Systems Secretion Primer (molecular biology) Molecular Biology 030304 developmental biology |
| Zdroj: | Molecular microbiologyREFERENCES. 115(3) |
| ISSN: | 1365-2958 |
| Popis: | "CryoEM" has come of age, enabling considerable structural insights into many facets of molecular biology. Here, we present a primer for microbiologists to understand the capabilities and limitations of two complementary cryoEM techniques for studying bacterial secretion systems. The first, single particle analysis, determines the structures of purified protein complexes to resolutions sufficient for molecular modeling, while the second, electron cryotomography and subtomogram averaging, tends to determine more modest resolution structures of protein complexes in intact cells. We illustrate these abilities with examples of insights provided into how secretion systems work by cryoEM, with a focus on type III secretion systems. |
| Databáze: | OpenAIRE |
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