Cytomegalovirus Monitoring by Polymerase Chain Reaction of Whole Blood Samples from Patients Undergoing Autologous Bone Marrow or Peripheral Blood Progenitor Cell Transplantation
| Autor: | F Gerneth, A. Schröder, Holger Hebart, B. Wassmann, Lothar Kanz, Jürgen Löffler, Hermann Einsele, Hans Martin, Thomas Klingebiel, H. Rabenau, Gerhard Jahn, C. A. Müller |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Human cytomegalovirus Allogeneic transplantation Adolescent Congenital cytomegalovirus infection Cytomegalovirus medicine.disease_cause Polymerase Chain Reaction Herpesviridae Blood Transfusion Autologous Cytopathogenic Effect Viral Predictive Value of Tests Risk Factors Betaherpesvirinae medicine Humans Immunology and Allergy Child Cells Cultured Bone Marrow Transplantation Whole blood biology business.industry Incidence virus diseases Fibroblasts Middle Aged Viral Load medicine.disease biology.organism_classification Transplantation Infectious Diseases medicine.anatomical_structure Child Preschool Cytomegalovirus Infections DNA Viral Immunology Bone marrow business |
| Zdroj: | The Journal of Infectious Diseases. 175:1490-1493 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/516484 |
| Popis: | Sensitive screening for cytomegalovirus (CMV) by polymerase chain reaction (PCR) following autologous bone marrow or peripheral blood progenitor cell transplantation has not been evaluated. In a three-center study, 98 autograft transplant recipients were prospectively screened for CMV infection by PCR and culture techniques. At a median of 20 days (range, 3 -28) after transplantation, 21 (39.6%) of 53 CMV-seronegative patients were PCR positive for CMV, and at a median of 17 days (range, 7-84) after transplantation, 19 (42.2%) of 45 CMV-seropositive patients were PCR positive for CMV. Low-level DNAemia (1-10 fg CMV DNA/mL blood) occurred for 1 week in 31 patients but was never associated with CMV disease. Of 9 patients who presented with at least two consecutive positive PCR results, I developed CMV pneumonia. No patients died because of CMV disease. Screening for CMV infection by PCR had a negative predictive value of 100% (as also observed after allogeneic transplantation), but its positive predictive value was significantly lower. |
| Databáze: | OpenAIRE |
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