A Type VI Secretion System of Pseudomonas aeruginosa Targets a Toxin to Bacteria
| Autor: | Rachel D. Hood, Wenzhuo Y. Wang, Rex R.S. Trinidad, Alexey J. Merz, Mike A. Carl, Joseph D. Mougous, FoSheng Hsu, David R. Goodlett, Tüzün Güvener, Sandra Schwarz, Pragya Singh, Rachael L. Plemel, Julie M. Silverman, Kevin G. Hicks, Brooks B. Ohlson, Mo Li |
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| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
MICROBIO Genomic Islands Mutant Bacterial Toxins Colony Count Microbial medicine.disease_cause Microbiology Article Evolution Molecular 03 medical and health sciences Bacterial Proteins Immunity Virology Immunology and Microbiology(all) Antibiosis Gene Order medicine Secretion Bacteriophages Molecular Biology 030304 developmental biology Type VI secretion system 0303 health sciences biology 030306 microbiology Pseudomonas aeruginosa Toxin Membrane Transport Proteins biology.organism_classification Anti-Bacterial Agents Parasitology Bacteria |
| Zdroj: | Cell Host & Microbe. 7(1):25-37 |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2009.12.007 |
| Popis: | SummaryThe functional spectrum of a secretion system is defined by its substrates. Here we analyzed the secretomes of Pseudomonas aeruginosa mutants altered in regulation of the Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS). We identified three substrates of this system, proteins Tse1–3 (type six exported 1–3), which are coregulated with the secretory apparatus and secreted under tight posttranslational control. The Tse2 protein was found to be the toxin component of a toxin-immunity system and to arrest the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cells; however, it provided a major growth advantage for P. aeruginosa strains, relative to those lacking immunity, in a manner dependent on cell contact and the H1-T6SS. This demonstration that the T6SS targets a toxin to bacteria helps reconcile the structural and evolutionary relationship between the T6SS and the bacteriophage tail and spike. |
| Databáze: | OpenAIRE |
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