Expression and localization of sterol 27-hydroxylase (CYP27A1) in monkey retina
| Autor: | Hirotoshi Fuda, Norman B. Javitt, Charles A. Strott, Ignacio R. Rodriguez, Jung Wha Lee |
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| Rok vydání: | 2006 |
| Předmět: |
Context (language use)
Biology Hydroxylation Article Retina Cellular and Molecular Neuroscience CYP27A1 medicine Animals Enzyme Inhibitors Pigment Epithelium of Eye Ketocholesterols Cells Cultured chemistry.chemical_classification Biological activity Immunohistochemistry Macaca mulatta eye diseases Sensory Systems Sterol Staining Cell biology Ophthalmology Enzyme medicine.anatomical_structure chemistry Biochemistry Steroid Hydroxylases Cholestanetriol 26-Monooxygenase sense organs Photoreceptor Cells Vertebrate |
| Zdroj: | Experimental Eye Research. 83:465-469 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2005.11.018 |
| Popis: | Sterol 27-hydroxylase (CYP27A1) is a mitochondrial P-450 enzyme with broad substrate specificity for C27 sterols including 7-ketocholesterol (7kCh). CYP27A1 is widely expressed in human tissues but has not been previously demonstrated in the retina. In this study, we examined the expression and localization of CYP27A1 in the monkey retina where it localized mainly to the photoreceptor inner segments. CYP27A1 was also observed in Müller cells with faint immuno staining detected in the RPE and choriocapillaris. We also determined that the 27-hydroxylation of 7-ketocholesterol (27OH7kCh) rendered it non-toxic to cultured RPE cells. Moreover, the 27OH7kCh when mixed with 7-ketocholesterol significantly reduced the toxicity of 7-ketocholesterol. These data, when taken in context of the known functions of CYP27A1 imply that expression in the retina serves to modify the biological activity of oxidized sterols that are either transported or generated locally by photo-oxidation. Published by Elsevier Ltd. |
| Databáze: | OpenAIRE |
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