Additional file 1: of Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

Autor: Aida, Tomomi, Nakade, Shota, Sakuma, Tetsushi, Izu, Yayoi, Ayu Oishi, Mochida, Keiji, Ishikubo, Harumi, Usami, Takako, Aizawa, Hidenori, Yamamoto, Takashi, Kohichi Tanaka
Rok vydání: 2016
DOI: 10.6084/m9.figshare.c.3629342_d1.v1
Popis: The following additional data are available with the online version of this paper. Additional data file 1 contains the figures including IDA analysis of PITCh-donor, PCR screenings of mice, sequencing of non-knock-in Actb alleles, FACS and LSM analysis of human cells, Exo1 western blotting, Exo1 toxicity analysis, off-target analysis in mice, germline transmission, linear PCR donor injection, and sequence alignments of hACTB and its off-target sites, and tables of these results and a list of the oligo DNAs and RNAs used in this study. (DOCX 14447 kb)
Databáze: OpenAIRE