Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance
| Autor: | Shi-Zhang Sun, Johanna M. Rommens, John W. Hanrahan, Christine E. Bear, Norbert Kartner, Cameron Ackerley, A. Leonard Naismith, Lap-Chee Tsui, Evangelica F. Reyes, John R. Riordan, Tim J. Jensen |
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| Rok vydání: | 1991 |
| Předmět: |
Anions
congenital hereditary and neonatal diseases and abnormalities Insecta Cystic Fibrosis Genetic Vectors Heterologous Cystic Fibrosis Transmembrane Conductance Regulator Sf9 Biology Transfection General Biochemistry Genetics and Molecular Biology Epitope Ion Channels Cell Line Membrane Potentials Chloride Channels Gene expression Cyclic AMP Animals Humans Patch clamp Cell Membrane Electric Conductivity Membrane Proteins Immunogold labelling respiratory system respiratory tract diseases Cell biology Kinetics Immunology biology.protein Antibody Baculoviridae Intracellular |
| Zdroj: | Cell. 64(4) |
| ISSN: | 0092-8674 |
| Popis: | The nature of involvement of the cystic fibrosis gene product (CFTR) in epithelial anion transport is not yet understood. We have expressed CFTR in Sf9 insect cells using the baculovirus expression vector system. Reactivity with antibodies against 12 different epitopes spanning the entire sequence suggested that the complete polypeptide chain was synthesized. Immunogold labeling showed localization to both cell-surface and intracellular membranes. Concomitant with CFTR expression, these cells exhibited a new cAMP-stimulated anion permeability. This conductance, monitored both by radioiodide efflux and patch clamping, strongly resembled that present in several CFTR-expressing human epithelial cells. These findings demonstrate that CFTR can function in heterologous nonepithelial cells and lend support to the possibility that CFTR may itself be a regulated anion channel. |
| Databáze: | OpenAIRE |
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