The expression of phospho-AKT1 and phospho-MTOR is associated with a favorable prognosis independent of PTEN expression in intrahepatic cholangiocarcinomas
| Autor: | Kwan Yong Choi, Hyung Jin Cha, Chang Ohk Sung, D.W. Choi, Dae Shick Kim, Dakeun Lee, Jin Young Park, Jongmin Kim, Jin Seok Heo, Jae-Won Joh, Kyusam Choi, Kee-Taek Jang, Seoung Ho Choi, In-Gu Do |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Blotting Western AKT1 Cell Cycle Proteins Kaplan-Meier Estimate Biology medicine.disease_cause Pathology and Forensic Medicine Cholangiocarcinoma Young Adult Republic of Korea Biomarkers Tumor medicine Humans Tensin PTEN Phosphorylation PI3K/AKT/mTOR pathway Intrahepatic Cholangiocarcinoma Adaptor Proteins Signal Transducing Aged Proportional Hazards Models Chi-Square Distribution TOR Serine-Threonine Kinases PTEN Phosphohydrolase Ribosomal Protein S6 Kinases 70-kDa Middle Aged Phosphoproteins Prognosis Immunohistochemistry Molecular biology Up-Regulation Bile Ducts Intrahepatic Bile Duct Neoplasms Ribosomal protein s6 embryonic structures biology.protein Cancer research Female Carcinogenesis Proto-Oncogene Proteins c-akt |
| Zdroj: | Modern Pathology. 25:131-139 |
| ISSN: | 0893-3952 |
| Popis: | AKT1 signaling pathway is important for the regulation of protein synthesis and cell survival with implications in carcinogenesis. In this study, we explored the prognostic significance of AKT1 pathway in intrahepatic cholangiocarcinomas. We investigated the status of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), phosphorylated (p) AKT1 (p-AKT1), p-mammalian target of rapamycin (p-MTOR), p-p70 ribosomal protein S6 kinase (p-RPS6KB2) and p-eukaryotic initiation factor 4E-binding protein-1 (p-EIF4EBP1) in 101 intrahepatic cholangiocarcinomas by immunohistochemistry. Western blot analysis was performed to verify the expression levels of p-AKT1 and p-MTOR. The relationship of protein expression with clinicopathological data and the correlations of protein expression levels were explored. The overexpression of p-AKT1, p-MTOR, and PTEN was associated with a better survival in patients with intrahepatic cholangiocarcinoma (P=0.0137, 0.0194, and 0.0337, respectively). In a multivariate analysis, PTEN was an independent prognostic factor, and p-AKT1 showed tendency (P=0.032 and 0.051, respectively). The overexpression of p-MTOR was correlated with well-to-moderately differentiated tumors (P |
| Databáze: | OpenAIRE |
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