Salt is Necessary for Nucleosomal DNA Fragmentation Induced by Caspase
| Autor: | Song Yub Shin, Changhyun Seong, Yun-Hee Lee, Byung Yun Cho, Jung Sup Lee, Bo-Eun Kim, Il-Seon Park |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
ICAD
Apoptosis Bioengineering DNA Fragmentation Cysteine Proteinase Inhibitors Sodium Chloride Applied Microbiology and Biotechnology Potassium Chloride Jurkat Cells chemistry.chemical_compound Animals Humans Fragmentation (cell biology) Poly-ADP-Ribose Binding Proteins Caspase Cell Nucleus Deoxyribonucleases biology Caspase 3 Aurintricarboxylic Acid Apoptotic DNA fragmentation Proteins General Medicine Caspase Inhibitors Molecular biology Chromatin Nucleosomes Rats chemistry Caspases Mutation Chromatography Gel biology.protein DNA fragmentation Salts Apoptosis Regulatory Proteins Deoxyribonuclease I Oligopeptides DNA Biotechnology |
| Zdroj: | Biotechnology Letters. 27:1935-1942 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-005-3906-z |
| Popis: | For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s). |
| Databáze: | OpenAIRE |
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