Pigment cell differentiation in embryonic mouse skin and isolated epidermis: an in vitro study
| Autor: | Leroy Oddis, Thomas C. Mayer |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Mice
Inbred Strains Skin Pigmentation Melanocyte Biology Pigment cell differentiation Mice Dermis Melanoblast medicine Animals Cells Cultured Cell Aggregation Skin integumentary system Epidermis (botany) Cell Differentiation General Medicine Embryonic stem cell W Series Cell biology medicine.anatomical_structure Epidermal Cells Cell culture Mutation Melanocytes Animal Science and Zoology |
| Zdroj: | The Journal of experimental zoology. 202(3) |
| ISSN: | 0022-104X |
| Popis: | Pigment cell differentiation was studied in cell cultures derived from 14-day embryonic mouse skin, and in cultures of dermal or epidermal skin cells. Cell cultures from dissociated whole skin produced melanocytes in very small numbers, and they were always present in association with groups of epithelial-like cells. Cell cultures from dissociated dermis produced no melanocytes during the three week culture period. Epidermal cultures established from mechanically produced small aggregates of cells first formed melanocytes after 30 hours in culture, and large numbers of melanocytes were present after two days. Epidermal cells, in contrast to other cells, did not survive in culture beyond 11 days, and consequently surviving populations of pure melanocytes were left remaining on the plate surface. Epidermal cultures prepared from albino embryos resulted in amelanotic melanocyte populations remaining following the epidermal cell death. Epidermal cell cultures from white-spotting mutant embryos (W/W and S1/S1) left no cells on the plate recognizable as being of the pigment cell lineage. The enhancement of melanoblast differentiation by epidermal cells, and the relationship between melanocytes and epidermal cells revealed in cell culture may be useful in the analysis of early stages of melanoblast differentiation, and in the study of mechanisms of gene action involved in this process. |
| Databáze: | OpenAIRE |
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