Dairy-inspired coatings for bone implants from whey protein isolate-derived self-assembled fibrils

Autor: Jenny Aveyard, Ute Hempel, Julia K. Keppler, Timothy E.L. Douglas, Rebecca Rabe, Laurine Martocq
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Whey protein isolate
02 engineering and technology
bone
lcsh:Chemistry
Coating
Osteogenesis
lcsh:QH301-705.5
Spectroscopy
Fibril
chemistry.chemical_classification
Stem cell
biology
fibril
Bone implant
Biomaterial
coating
Cell Differentiation
General Medicine
021001 nanoscience & nanotechnology
Milk Proteins
Computer Science Applications
0210 nano-technology
Stromal cell
macromolecular substances
engineering.material
Catalysis
Article
Self assembled
Inorganic Chemistry
03 medical and health sciences
Humans
Physical and Theoretical Chemistry
Bone
Food Process Engineering
Molecular Biology
Cell Proliferation
Bone Development
Osteoblasts
Biomolecule
Organic Chemistry
whey protein isolate
Mesenchymal Stem Cells
stem cell
030104 developmental biology
Whey Proteins
lcsh:Biology (General)
lcsh:QD1-999
Chemical engineering
chemistry
biology.protein
engineering
Adsorption
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences 21 (2020) 15
Volume 21
Issue 15
International Journal of Molecular Sciences, 21(15)
International Journal of Molecular Sciences, Vol 21, Iss 5544, p 5544 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21155544
Popis: To improve the integration of a biomaterial with surrounding tissue, its surface properties may be modified by adsorption of biomacromolecules, e.g., fibrils. Whey protein isolate (WPI), a dairy industry by-product, supports osteoblastic cell growth. WPI&rsquo
s main component, &beta
lactoglobulin, forms fibrils in acidic solutions. In this study, aiming to develop coatings for biomaterials for bone contact, substrates were coated with WPI fibrils obtained at pH 2 or 3.5. Importantly, WPI fibrils coatings withstood autoclave sterilization and appeared to promote spreading and differentiation of human bone marrow stromal cells (hBMSC). In the future, WPI fibrils coatings could facilitate immobilization of biomolecules with growth stimulating or antimicrobial properties.
Databáze: OpenAIRE
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